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Complete HPLC and LC-MS

17 - 21 November 2025, Milton Keynes, United Kingdom


Introduction
The Complete LC & LC-MS course is built from the Practical Essentials of LC & LC-MS and the LC & LC-MS Clinic courses to provide a complete training solution enabling you to understand your Liquid Chromatograph or LC-MS instrument, application method development, troubleshooting & maintenance.

This course covers all the practical theory you need to know about liquid chromatography and its related techniques including liquid chromatography-mass spectrometry. Mobile phase optimisation including gradient elution, pumps and plumbing, autosampler injection techniques, sample preparation, calibration standards, data analysis for quantitation and qualitative analysis are discussed.

It also looks at how to develop appropriate application specific HPLC & LC-MS methods and the set-up and optimisation of instrumentation. Finally the steps required to maintain and troubleshoot the system, problem chromatography and mass spectra are covered. Advanced techniques are discussed and their application to difficult samples requiring optimal limits of detection and linear dynamic range. HPLC to UHPLC method transfer is touched upon, chromatographic deconvolution utilising tandem mass spectrometry, multi-dimensional LC (LCxLC), on-line SPE and chemometrics.

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Speakers
Venue
The Open University

The Open University , Walton Hall, Milton Keynes, MK7 6AA, United Kingdom

Organised by
Anthias Consulting Ltd
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