欧美AV

What will you learn on this course? This course provides the theory of HPLC and LC-MS essential to any participant, along with the hands-on practical element in the laboratory to practice and re-enforce the theoretical knowledge. These courses are 50% theory and 50% practical in a lab environment. After an introduction to liquid chromatography, this course addresses solvents and buffers (the mobile phase), pumps, isocratic and gradient elution, sample introduction, analytical columns, HPLC detectors, mass spectrometry including tuning, ionisation, mass analysers, ion detector and data analysis. The knowledge is then used to create methods, perform injections and change parameters to see the effects. The course discusses the need for maintenance along with practicals to carry out maintenance on both an HPLC and an LC-MS system such as the autosampler, injector, columns, detectors, MS tuning, vacuum system, ion source and the mass analyser. A day is spent on troubleshooting an HPLC and LC-MS instrument and learning what problems can occur, with real problem solving on the instruments and data analysis software. This course combines the five individual hands-on days coveringHPLC Theory and Methods,HPLC Maintenance,LC-MS Theory and Methods,LC-MS MaintenanceandHPLC and LC-MS Troubleshooting. Learning outcomes

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You will learn the importance of good chromatography

You will learn about the function and operation of the individual HPLC components

You will become familiar with the HPLC instrumentation

You will have practical experience of adjusting the parameters for each HPLC component

You will have practical experience of performing maintenance on each HPLC component

You will learn the principles of mass spectrometry

You will learn about the function and operation of the LC-MS individual components

You will become familiar with the MS instrumentation

You will have practical experience of adjusting the parameters for each MS component

You will have practical experience of performing maintenance on each MS component

You will learn the strategies for HPLC and LC-MS instrument troubleshooting

You will have practical experience of HPLC and LC-MS instrument troubleshooting

Who is this course for? This course is suitable for all levels of knowledge; however for complete beginners it is advisable to attend theAbsolute Basics of HPLC and LC-MSfirst to get a good basic overview of liquid chromatography and LC-MS."