This week long course covers how to perform a metabolic profiling experiment from start to finish. It will cover study design, sample preparation, the use of mass spectrometry for global profiling, targeted methodologies and data analysis.
Day 1: Introductory lectures in mass spectrometry and chromatography, study design and sample preparation, followed by preparation of biological samples for analysis on subsequent days.
Days 2 and 3: Analysis of biofluids through global profiling and targeted analyses; an introductory session to liquid chromatography, followed by sessions on each of the newest QToF and TQ instrumentation. Instrument set up, method development and acquisition will be covered. We have set a maximum of 3 attendees per instrument allowing for hands-on participation by all. Day 3 finishes with introduction to data analysis.
Day 4: Data analysis workshops where attendees will process the data acquired from the previous days, mixed with further statistics lectures, allowing for development of interpretation skills.
Day 5: Application lectures, tips, tricks and troubleshooting, optional trip to the MRC-NIHR National Phenome Centre.
Day 1: Introductory lectures in mass spectrometry and chromatography, study design and sample preparation, followed by preparation of biological samples for analysis on subsequent days.
Days 2 and 3: Analysis of biofluids through global profiling and targeted analyses; an introductory session to liquid chromatography, followed by sessions on each of the newest QToF and TQ instrumentation. Instrument set up, method development and acquisition will be covered. We have set a maximum of 3 attendees per instrument allowing for hands-on participation by all. Day 3 finishes with introduction to data analysis.
Day 4: Data analysis workshops where attendees will process the data acquired from the previous days, mixed with further statistics lectures, allowing for development of interpretation skills.
Day 5: Application lectures, tips, tricks and troubleshooting, optional trip to the MRC-NIHR National Phenome Centre.
